Western blot of G8965-07. Lane 1: Molecular Weight Marker. Lane 2: GFP protein (50ng). Primary Antibody: Anti-GFP at 1:1000 overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG HRP at 1:40,000 for 30mins at RT. Expected MW: ~27kD

Western Blot of G8965-07. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. Primary antibody: GFP antibody at 1mg/ml for 1 h at room temperature. Secondary antibody: conjugated Goat-a-Mouse IgG [H&L] at 1:2,500 dilution for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): none.